An optical density greater than zero indicates

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Multiple Choice

An optical density greater than zero indicates

Explanation:
An optical density reading above zero means the BAPNA substrate has been cleaved by the protease to produce a colored product that the spectrophotometer detects. In this setup, pepsin is the enzyme acting on BAPNA at acidic pH, so a positive reading indicates both that digestion has occurred and that pepsin was active under the conditions used. If pepsin weren’t active or digestion hadn’t occurred, no color would form and the OD would stay at zero.

An optical density reading above zero means the BAPNA substrate has been cleaved by the protease to produce a colored product that the spectrophotometer detects. In this setup, pepsin is the enzyme acting on BAPNA at acidic pH, so a positive reading indicates both that digestion has occurred and that pepsin was active under the conditions used. If pepsin weren’t active or digestion hadn’t occurred, no color would form and the OD would stay at zero.

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