What is the substrate used to detect protein digestion in this activity?

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Multiple Choice

What is the substrate used to detect protein digestion in this activity?

Explanation:
Protein digestion is monitored using a substrate that releases a detectable color when a protease cuts it. BAPNA, or N-α-benzoyl-D-arginine p-nitroanilide, is such a substrate. When a protease cleaves the peptide bond in BAPNA, it releases p-nitroaniline, which turns yellow and can be measured spectrophotometrically. This color change provides a direct readout of proteolytic activity and how much digestion has occurred. The other options aren’t used for this purpose: starch is a carbohydrate, Benedict’s solution detects reducing sugars, and pepsin is the enzyme, not the substrate used to report digestion.

Protein digestion is monitored using a substrate that releases a detectable color when a protease cuts it. BAPNA, or N-α-benzoyl-D-arginine p-nitroanilide, is such a substrate. When a protease cleaves the peptide bond in BAPNA, it releases p-nitroaniline, which turns yellow and can be measured spectrophotometrically. This color change provides a direct readout of proteolytic activity and how much digestion has occurred. The other options aren’t used for this purpose: starch is a carbohydrate, Benedict’s solution detects reducing sugars, and pepsin is the enzyme, not the substrate used to report digestion.

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