Which tube contains pepsin with BAPNA at neutral pH?

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Multiple Choice

Which tube contains pepsin with BAPNA at neutral pH?

Explanation:
Pepsin requires an acidic environment to be active; at neutral pH its proteolytic site isn’t properly formed, so it can’t efficiently cleave substrates. BAPNA is used as a substrate that releases a color when a protease cleaves it, so you’d see color development only if pepsin is active. Therefore the tube that contains pepsin and BAPNA at neutral pH will show little to no color change, reflecting the lack of pepsin activity under those conditions. This is why that neutral-pH tube is the correct choice. Tubes at acidic pH would promote pepsin activity and show color change if BAPNA is cleaved, while tubes without pepsin would also show little color change regardless of pH.

Pepsin requires an acidic environment to be active; at neutral pH its proteolytic site isn’t properly formed, so it can’t efficiently cleave substrates. BAPNA is used as a substrate that releases a color when a protease cleaves it, so you’d see color development only if pepsin is active. Therefore the tube that contains pepsin and BAPNA at neutral pH will show little to no color change, reflecting the lack of pepsin activity under those conditions. This is why that neutral-pH tube is the correct choice. Tubes at acidic pH would promote pepsin activity and show color change if BAPNA is cleaved, while tubes without pepsin would also show little color change regardless of pH.

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